Optometry & Visual Performance 177 Volume 14 | Issue 2 | June 2026 RNFL Thickness Strabismic Amblyopia Anisometropic Amblyopia Bilateral Amblyopia Meridional Amblyopia F P-value Mean SD Mean SD Mean SD Mean SD Nasal Quadrant 80.82 12.17 85.82 20.77 94.65 24.46 81.20 20.42 1.792 0.15 Temporal Quadrant 69.88 9.84 75.67 14.53 68.09 13.61 75.20 9.83 2.266 0.86 SupraTemporal Quadrant 132.63 20.56 135.38 16.47 133.84 21.84 133.60 11.78 0.071 0.97 SupraNasal Quadrant 142.38 25.66 121.05 30.23 122.23 27.53 125.80 23.67 1.313 0.27 InferoNasal Quadrant 150.13 27.28 126.18 28.52 136.37 25.44 148.00 50.79 2.343 0.078 InferoTemporal Quadrant 139.75 23.87 143.92 17.16 145.07 26.05 140.60 10.04 0.173 0.915 Globally 108.13 14.11 105.74 11.04 107.84 12.32 105.80 12.65 0.251 0.860 Table 3. RNFL Thickness in Different Types of Amblyopia RNFL Thickness Amblyopic Eyes Non-amblyopic Eyes T P-value Mean SD Mean SD Nasal Quadrant 86.89 18.70 82.80 17.46 -1.275 0.209 Temporal Quadrant 75.87 14.17 74.27 10.54 -0.949 0.348 SupraTemporal Quadrant 140.89 19.21 138.81 18.73 -0.791 0.433 SupraNasal Quadrant 132.57 27.07 129.47 27.85 -0.970 0.337 InferoNasal Quadrant 139.13 32.17 136.87 32.27 -0.750 0.457 InferoTemporal Quadrant 147.18 22.15 146.11 20.74 -0.337 0.737 Globally 105.22 23.64 108.07 10.11 -0.877 0.385 Table 4. RNFL Thickness Between Amblyopic and NonAmblyopic Eyes The one-way ANOVA was used to compare the mean difference in RNFL thickness among different types of refractive errors. There was no statistically significant difference between various types of refractive error in all quadrants except supra-nasal. RNFL thickness for the supra-nasal quadrant in hyperopes was thicker amongst the refractive errors given (Table 5). The one-way ANOVA was also used to compare the mean difference in RNFL thickness among amblyopic patients with and without using patching therapy. No statistically significant difference was found in all quadrants, as shown in Table 6. Discussion The main objective of this study was to determine the RNFL thickness and compare it between different types of amblyopia and fellow non-amblyopic eyes. In the present study, the mean RNFL thickness was not statistically significantly different between amblyopic and non-amblyopic eyes, which is in accordance with the study conducted by Kee et al.,15 in which the thicknesses of the superior, inferior, nasal, and temporal quadrants of the retinal nerve fiber layer between normal children and children with amblyopia were also not statistically significant. In a study by Yen et al.,16 the difference in RNFL thickness between strabismic amblyopic eyes and fellow eyes did not reach statistical significance, so it was concluded that amblyopia is not linked with RNFL thickness. In thirty individuals with aniso-hypermetropic amblyopia, Yoon et al.17 used the OCT to evaluate both peripapillary and macular RNFL thickness. They found that anisometropic amblyopic eyes have thicker peripapillary RNFL than normal eyes. Because of the tiny size of the eye, those with high hypermetropia may have thicker RNFL, and the apparently increased thickness of RNFL may be attributable to this structural alteration in the eye rather than the effect of amblyopia.
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