Optometry & Visual Performance 179 Volume 14 | Issue 2 | June 2026 between the myopes vs. hyperopes and emmetropes was statistically significant (p<0.05) as compared to hyperopes vs. emmetropes (p=0.152). The mean values of RNFL thickness were thinner for myopes vs. hyperopes (p<0.05) in all quadrants. The mean value of RNFL thickness was greater in emmetropes than hyperopes in both the temporal and superior quadrants.20 The findings of the present study indicate that RNFL thickness for the supra-nasal quadrant in hyperopic amblyopia was thicker amongst the refractive errors. There was no statistically significant difference between various types of refractive error in other quadrants. Conclusion This study offers a significant and surprising insight into the relationship between RNFL thickness and amblyopia. Our results contradict the widely held belief that identifiable structural alterations in the RNFL are a consequence of amblyopia, a disorder generally linked to aberrant visual development. The RNFL thickness of amblyopic eyes was found to be similar to that of their fellow (non-affected) eyes in all retinal quadrants. This held true regardless of the type of amblyopia. These findings directly contradict the conventional belief that amblyopia leads to thinning of the RNFL, which has been thought to reflect underlying retinal or optic nerve pathology. Our study suggests that the structural integrity of the RNFL may remain unaffected in amblyopia, even in the presence of significant visual deficits. This calls for assessing the existing theories of the pathophysiology of amblyopia, especially with regard to its assumed impact on retinal structure. References 1. Al-Tamimi ER, Shakeel A, Yassin SA, Ali SI, Khan UA. 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